diff --git a/ports/esp32/boards/ARDUINO_NANO_ESP32/board_init.c b/ports/esp32/boards/ARDUINO_NANO_ESP32/board_init.c
index 69a414cd5d..3394bb1e2e 100644
--- a/ports/esp32/boards/ARDUINO_NANO_ESP32/board_init.c
+++ b/ports/esp32/boards/ARDUINO_NANO_ESP32/board_init.c
@@ -35,9 +35,6 @@
 #include "double_tap.h"
 #include "usb.h"
 
-#include "tinyusb.h"
-#include "tusb_cdc_acm.h"
-
 #define LED_RED     GPIO_NUM_46
 #define LED_GREEN   GPIO_NUM_0
 #define LED_BLUE    GPIO_NUM_45
@@ -87,11 +84,6 @@ void NANO_ESP32_enter_bootloader(void) {
     esp_restart();
 }
 
-void NANO_ESP32_usb_callback_line_state_changed(int itf, void *event_in) {
-    cdcacm_event_t *event = event_in;
-    mp_usbd_line_state_cb(itf, event->line_state_changed_data.dtr, event->line_state_changed_data.rts);
-}
-
 void NANO_ESP32_board_startup(void) {
     boardctrl_startup();
 
diff --git a/ports/esp32/boards/ARDUINO_NANO_ESP32/mpconfigboard.h b/ports/esp32/boards/ARDUINO_NANO_ESP32/mpconfigboard.h
index 8c2aa88e9f..31b2a49bf8 100644
--- a/ports/esp32/boards/ARDUINO_NANO_ESP32/mpconfigboard.h
+++ b/ports/esp32/boards/ARDUINO_NANO_ESP32/mpconfigboard.h
@@ -19,14 +19,9 @@
 #define MICROPY_HW_SPI2_SCK                 (18)
 
 #define MICROPY_HW_ENABLE_USBDEV            (1)
-#define MICROPY_EXCLUDE_SHARED_TINYUSB_USBD_CDC (1)
-#define MICROPY_HW_USB_EXTERNAL_TINYUSB     (1)
 #define MICROPY_HW_USB_CDC_1200BPS_TOUCH    (1)
 #define MICROPY_SCHEDULER_STATIC_NODES      (1)
 
-#define MICROPY_HW_USB_CUSTOM_LINE_STATE_CB             NANO_ESP32_usb_callback_line_state_changed
-void NANO_ESP32_usb_callback_line_state_changed(int itf, void *event);
-
 #define MICROPY_BOARD_STARTUP                           NANO_ESP32_board_startup
 void NANO_ESP32_board_startup(void);